Procedure

3AP Lesion

Annealing DNA

Colony PCR

Chloroform Extraction & EtOH Precipitation

Maxi-Prep

Confocal Microscope

Transgene Preparation

Densitometry

Dox Administration

Immuflourescence Slides

Cell culture immunoflourescence

Brain Immunoflourescence

DAPI Staining (Nuclear)

Isolation Tail DNA

Mounting Slides

Southern Blot

Agarace Gel Extraction

Transformation and Standard Ligation

siRNA Transfection

Transfection with Lipofectamine

Insoluble/Soluble Fractionation

Nuclear/Cytoplamsic Fractionation

Immunoprecipitation

Western Blot (Cell culture collection, Bradford assay)

Bradford Template

Western blot of cerebellar tissue

Fascist regime

Western Blot Protocol (Probing)

Western Blot stripping

Flourescence

RNA Extraction

Northern/Southern Stripping

Northern Blot

Half-life

dNTPS

Standard Solutions